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bca protein assay kit beyotime p0010 nadp nadph assay kit beyotime s0179 mitochondrial membrane potential assay kit  (Beyotime)


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    Beyotime bca protein assay kit beyotime p0010 nadp nadph assay kit beyotime s0179 mitochondrial membrane potential assay kit
    Bca Protein Assay Kit Beyotime P0010 Nadp Nadph Assay Kit Beyotime S0179 Mitochondrial Membrane Potential Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 798 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bca+protein+assay+kit+p0010/Enhanced+mitochondrial+membrane+potential+assay+kit+with+JC-1/pm41105514-484-225-229
    Average 99 stars, based on 798 article reviews
    bca protein assay kit beyotime p0010 nadp nadph assay kit beyotime s0179 mitochondrial membrane potential assay kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Lysis:

    Article Title: "TFEB - HNRNPA2B1": A Positive Feedback Loop Facilitating Metastasis in Hepatocellular Carcinoma cells.
    Article Snippet: This study elucidated the regulatory role of the TFEB-HNRNPA2B1 feedback loop in hepatocellular carcinoma (HCC) metastasis, a highly prevalent and aggressive liver cancer subtype.. The findings demonstrated that transcription factor EB (TFEB), a master regulator of lysosomal biogenesis, was significantly upregulated in HCC tissues, particularly in advanced-stage tumors and lymph node metastatic lesions.. Functional studies revealed that TFEB overexpression enhanced metastatic potential in HCC cell lines, whereas its genetic knockdown substantially suppressed metastatic capacity.

    Bicinchoninic Acid Protein Assay:

    Article Title: "TFEB - HNRNPA2B1": A Positive Feedback Loop Facilitating Metastasis in Hepatocellular Carcinoma cells.
    Article Snippet: This study elucidated the regulatory role of the TFEB-HNRNPA2B1 feedback loop in hepatocellular carcinoma (HCC) metastasis, a highly prevalent and aggressive liver cancer subtype.. The findings demonstrated that transcription factor EB (TFEB), a master regulator of lysosomal biogenesis, was significantly upregulated in HCC tissues, particularly in advanced-stage tumors and lymph node metastatic lesions.. Functional studies revealed that TFEB overexpression enhanced metastatic potential in HCC cell lines, whereas its genetic knockdown substantially suppressed metastatic capacity.

    Article Title: Elevated SGO2 expression in lung adenocarcinoma promotes migration and invasion via MAD2 and correlates with poor prognosis.
    Article Snippet: .. The protein concentration was quantified utilizing a BCA protein assay kit (P0010, Beyotime, Shanghai). .. The cell lysis was performed on ice for a duration of 10 minutes using RIPA buffer (R0010, Solarbio, Beijing).

    Article Title: A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming.
    Article Snippet: Cells were lysed in RIPA buffer containing protease and phosphatase inhibitors (P1049, Beyotime). .. Protein concentration was measured using the BCA protein assay kit (P0010, Beyotime). .. Equal amounts of protein (30 μg) were separated by SDSPAGE and transferred onto PVDF membranes (ISEQ00010, Merck millipore).

    Article Title: SIRT3 mediates CPT2 delactylation to enhance mitochondrial function and proliferation in goat granulosa cells
    Article Snippet: In some experiments, the lysis buffer also contained a mixture of deacetylase inhibitors (P1112, Beyotime, Shanghai, China), and all steps were performed at 4 °C. .. The protein concentration was measured using a BCA protein assay kit (P0010, Beyotime, Shanghai, China). .. The protein samples were mixed with 5 × loading buffer (BL502, Biosharp, Hefei, China) and denatured by boiling at 100 °C for 5‒10 min.

    Article Title: Proteomics and cytokine array jointly reveal the role of macrophage proinflammatory shift in liver fibrosis in dairy cows with ketosis.
    Article Snippet: .. The total protein in the supernatant was quantified using a BCA protein assay kit (P0010, Beyotime, Shanghai, China) following the manufacturer’s instructions. ..

    Article Title: RDM1 plays an oncogenic role in clear cell renal cell carcinoma potentially by modulating MCM2.
    Article Snippet: .. The BCA protein assay kit (P0010, Beyotime Biotechnology) was employed in detecting protein contents. .. Proteins were loaded onto 10% SDS-PAGE gels (PG112, Epizyme Biotech) and separated through electrophoresis before transfer on 0.45-μm PVDF membranes (Millipore, USA).

    Article Title: Transcription factor TCF3 promotes bladder cancer development via TMBIM6-Ca 2+ -dependent ferroptosis.
    Article Snippet: Lipid ROS levels were then measured by flow cytometry (A00-1-1102, Beckman, USA). .. The BCA protein assay kit (P0010, Beyotime, China) was used to detect protein concentrations. ..

    Article Title: TIMM8B promotes oxidative phosphorylation and glycolysis by inhibiting the mtROS/ASK1/JNK signaling pathway in ovarian cancer.
    Article Snippet: Cell lysis solution (P0013, Beyotime) was used to extract total protein from the cells. .. After protein extraction, a BCA protein assay kit (P0010, Beyotime) was used for protein quantification, followed by the separation of the proteins via SDS-PAGE. .. The membranes were incubated with the following primary antibodies: TIMM8B (1:1000, abs152377, Absin), cyclin E1 (1:2000, AF0144, Affinity), p27 (1:1000, 3686S, Cell Signaling Technology), cyclin D1 (1:3000, ab134175, Abcam), p21 (1:1000, 14472S, Cell Signaling Technology), E-cadherin (1:1000, 14472S, Cell Signaling Technology), vimentin (1:1000, ab8978, Abcam), Snail (1:1000, ab216347, Abcam), N-cadherin (1:1000, 13116S, Cell Signaling Technology), BCL-XL (1:1000, AF6414, Affinity), BCL-2 (1:1000, AF6139, Affinity), BAX (1:2000, 29,552–1-AP, Proteintech), cleavedCaspase 3 (1:2000, AF0722, Affinity), cleaved-Caspase 7 (1:2000, AF4023, Affinity), NDUFS1 (Complex I, 1:1000, DF7041, Affinity), SDHB (Complex II, 1:1000, DF12732, Affinity), CYTB polyclonal (Complex III, 1:2000, 55,090– 1-AP, Proteintech); Complex IV (1:1000, 4850, Cell Signaling), ATP5A (Complex V, 1:1000, ab14748, Abcam), ASK1 (1:1000, 8662S, Cell Signaling Technology), p-JNK (1:1000, AF3318, Affinity), JNK (1:1000, AF6318, Affinity), and GAPDH (1:5000, AB0037, Abways) at 4 °C overnight.

    Protein Concentration:

    Article Title: Elevated SGO2 expression in lung adenocarcinoma promotes migration and invasion via MAD2 and correlates with poor prognosis.
    Article Snippet: .. The protein concentration was quantified utilizing a BCA protein assay kit (P0010, Beyotime, Shanghai). .. The cell lysis was performed on ice for a duration of 10 minutes using RIPA buffer (R0010, Solarbio, Beijing).

    Article Title: A dual-targeting strategy to inhibit colorectal cancer liver metastasis via tumor cell ferroptosis and cancer-associated fibroblast reprogramming.
    Article Snippet: Cells were lysed in RIPA buffer containing protease and phosphatase inhibitors (P1049, Beyotime). .. Protein concentration was measured using the BCA protein assay kit (P0010, Beyotime). .. Equal amounts of protein (30 μg) were separated by SDSPAGE and transferred onto PVDF membranes (ISEQ00010, Merck millipore).

    Article Title: SIRT3 mediates CPT2 delactylation to enhance mitochondrial function and proliferation in goat granulosa cells
    Article Snippet: In some experiments, the lysis buffer also contained a mixture of deacetylase inhibitors (P1112, Beyotime, Shanghai, China), and all steps were performed at 4 °C. .. The protein concentration was measured using a BCA protein assay kit (P0010, Beyotime, Shanghai, China). .. The protein samples were mixed with 5 × loading buffer (BL502, Biosharp, Hefei, China) and denatured by boiling at 100 °C for 5‒10 min.

    Protein Extraction:

    Article Title: TIMM8B promotes oxidative phosphorylation and glycolysis by inhibiting the mtROS/ASK1/JNK signaling pathway in ovarian cancer.
    Article Snippet: Cell lysis solution (P0013, Beyotime) was used to extract total protein from the cells. .. After protein extraction, a BCA protein assay kit (P0010, Beyotime) was used for protein quantification, followed by the separation of the proteins via SDS-PAGE. .. The membranes were incubated with the following primary antibodies: TIMM8B (1:1000, abs152377, Absin), cyclin E1 (1:2000, AF0144, Affinity), p27 (1:1000, 3686S, Cell Signaling Technology), cyclin D1 (1:3000, ab134175, Abcam), p21 (1:1000, 14472S, Cell Signaling Technology), E-cadherin (1:1000, 14472S, Cell Signaling Technology), vimentin (1:1000, ab8978, Abcam), Snail (1:1000, ab216347, Abcam), N-cadherin (1:1000, 13116S, Cell Signaling Technology), BCL-XL (1:1000, AF6414, Affinity), BCL-2 (1:1000, AF6139, Affinity), BAX (1:2000, 29,552–1-AP, Proteintech), cleavedCaspase 3 (1:2000, AF0722, Affinity), cleaved-Caspase 7 (1:2000, AF4023, Affinity), NDUFS1 (Complex I, 1:1000, DF7041, Affinity), SDHB (Complex II, 1:1000, DF12732, Affinity), CYTB polyclonal (Complex III, 1:2000, 55,090– 1-AP, Proteintech); Complex IV (1:1000, 4850, Cell Signaling), ATP5A (Complex V, 1:1000, ab14748, Abcam), ASK1 (1:1000, 8662S, Cell Signaling Technology), p-JNK (1:1000, AF3318, Affinity), JNK (1:1000, AF6318, Affinity), and GAPDH (1:5000, AB0037, Abways) at 4 °C overnight.



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